Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GABA-B receptor 2 in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GABA-B receptor 2 in human cerebellum and rat cerebral cortex tissue.

Anti-GABA-B receptor 2 Antibody, clone N81/37 is a highly specific mouse monoclonal antibody, that targets GABA B Receptor & has been tested in western blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants.

Immunogen

Recombinant protein corresponding to rat GABA-B receptor 2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABA-B receptor 2 in 10 µg of rat brain tissue lysate.

Specificity

This antibody does not cross react with GABABR1 (Prof. J. Trimmer, University of California, Davis).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~105 kDa observed